The actor, who has been repeatedly linked to Keralan actress Asin, posted that he was not in a relationship at present.
Mukesh wrote: "Yes I am single! If there was someone special I would have spoken about her.
"But sorry guys I got no love. So nothing to talk about. Yet (sic)."
The New York star had commented in 2009 that he planned to marry girlfriend Priyanka Bhatia, from whom he later separated.
The actor and Asin are expected to begin work on Pocketmaar later in 2011.
Mukesh has over 140,000 followers on the social networking site.