The film's title character, played by Kareena Kapoor, is seen being assaulted by her actor boyfriend.
A source told Mid Day: "Kangana is extremely close to Madhur [Bhandarkar, director of Heroine] and had worked with him in Fashion. However, she never expected that Madhur would recreate a scene from her real life and use it in the film.
"Although everyone from Madhur to the production house are not revealing anything about the scene, Kangana has heard that it is indeed the incident from her past life."
In 2009, Ranaut filed a complaint against her then boyfriend Aditya Pancholi after he allegedly attacked her.
However, controversial filmmaker Bhandarkar has denied that the film is based on any one actress's personal life.
The trailer for the film has received over 2 million views on YouTube since it was launched in July.
Watch the official trailer for Heroine below: