Experts believe the issue - a high grade copy of Detective Comics #27 - which is being sold with no reserve and no minimum bid, will fetch at least $300,000 at an auction run by Heritage Auction Galleries in America, on February 25.
The current record for a comic sold at auction stands at $317,000 for a copy of Action Comics #1, which featured the first appearance of Superman.
Lon Allen, director of sales for the comics division of Heritage, told website News-Antique.com: "Since Heritage began auctioning vintage comics, we have heard more client requests for Detective #27 than for Superman’s first comic, Action #1, and that’s both from hardcore comic collectors and from clients in other fields who are interested in this issue as a pop culture milestone.
"Superman came first, but I think in certain quarters Batman is the more popular character. This is probably the most desirable comic Heritage has ever auctioned.
"The eye appeal is absolutely outstanding. Most comics from 1939 are smudged, torn, creased and so on - this one’s got really bright colours and looks fresh. It has no restoration, which is a major plus as well. We’ve never handled a copy that’s anywhere near this nice."
Heritage has not disclosed the source of the comic other than to note it comes from a savvy collector who assembled his collection in the 1960s and 1970s.
Batman, originally referred to as the Bat-Man, was co-created by artist Bob Kane and writer Bill Finger.