The comic will see Mike Mignola's demonic hero team up with Evan Dorkin and Jill Thompson's magical pets.
Dorkin will pen the one-shot, which does not yet have a release date, while Thompson will provide painted interior art.
"I was pretty knocked out," Dorkin told CBR. "Obviously, [this was] not something I expected, and it was very flattering, and also something I was not sure would make sense.
"I gave it some thought - we were both obviously interested in the idea, for various reasons, some creative, some pragmatic. I'm a big Hellboy fan. I was pretty overwhelmed.
"Mike just basically gave us his blessing to use the character. That means a lot to us. And hopefully we'll do right by everybody - by his character, by his readers, and by our readers. Make it a neat comic book worth three bucks."
The Beasts of Burden first appeared in various Dark Horse anthologies before receiving their own critically-acclaimed miniseries last year.