The new series will be penned by Black Panther writer David Liss.
The announcement follows yesterday's revelation that Dynamite has acquired the rights to classic pulp property The Shadow.
"Pulp fiction is full of big characters, but few come bigger than the Spider - a rugged, daring, self-sacrificing character who would take any risk to face down enemies of New York and the nation," said Liss.
"And what enemies they were. The villains that filled the pages of The Spider killed thousands, destroyed whole portions of the city, infected people by the hundreds, released vermin, hypnotized, zombified and otherwise transformed anyone who came into their path."
Liss added: "Partly it's the sheer scale of the Spider's world that makes him so compelling, but it is also his own quiet, determined dignity in the face of villainous bombast, his rock-steady sanity as he confronts an insane world."
The Spider was created by Popular Publications' Harry Steeger in 1933.
Dynamite is known for its licensed properties, including Zorro and the forthcoming A Game of Thrones adaptation.