The video shows pages from Peter Milligan and Mikel Janin's forthcoming title, featuring scenes of devastation before introducing the cast of the book.
"When evil becomes madness and chaos spreads everywhere, heroes are not enough against magic," reads the trailer text. "Who will stand against darkness?"
Justice League Dark will feature supernatural characters Deadman, Zatanna, John Constantine, Madame Xanadu and Shade the Changing Man, the latter of whom was famously reimagined by Milligan in the early '90s.
Watch the trailer for Justice League Dark below:
"The witch known as The Enchantress has gone mad, unleashing forces that not even the combined powers of Superman, Batman, Wonder Woman and Cyborg can stop," reads DC's solicitation for issue #1.
"And if those heroes can't handle the job, who will stand against this mystical madness?
"Shade the Changing Man, Madame Xanadu, Deadman, Zatanna and John Constantine may be our only hope - but how can we put our trust in beings whose very presence makes ordinary people break out in a cold sweat?"
Constantine was recently reintroduced to the DC Universe after a long run solely within the publisher's isolated Vertigo imprint.
Justice League Dark #1 will be released on September 28.