The image for the 'Ends of the Earth' storyline shows the villain readying a punch, with Peter Parker - caught by the throat - seen reflected in his helmet.
This is the third teaser released this week for the story which sees the return of the Sinister Six and the revenge of Doctor Octopus.
The previous teasers featured Spider-Man being attacked by Otto Octavius and Electro.
Teasers released earlier in the month revealed various details of the storyline, including Spider-Man's new armoured costume.
> 'Amazing Spider-Man' sees Doctor Octopus return
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The storyline marks the 50th anniversary of Spider-Man.
'Ends of the Earth' will launch in Dan Slott and Stefano Caselli's Amazing Spider-Man #682, which will debut on March 21.