Peter Parker of the mainstream Marvel Universe has been revealed on the publisher's teaser.
The teaser was previously released with both characters blocked out.
New Ultimate Spider-Man star Miles Morales is expected to be the yet-to-be-revealed second star of the project.
Ultimate Comics Spider-Man collaborators Brian Michael Bendis and Sara Pichelli are collaborating on the series, which will arrive in June.
2012 marks the 50th anniversary of Spider-Man.
The first teaser for Spider-Men showed a fusion of the mainstream and 'Ultimate' Spider-Man logos, leading to speculation that the alternate reality heroes would collide in the title.