The art, featured on the cover of Hergé's book Tintin in America, fetched €1.3m (£1m/$1.6m) at a Paris auction on Saturday (June 2), according to The Hollywood Reporter.
Only five of the covers are known to exist, with two of them in private hands. The same artwork had set the previous record for comic book art when it sold in 2008 for £633,000 ($972,000).
The record for American comic art is held by a splash page from The Dark Knight Returns in 2011, which sold for $448,125 (£291,662).
The Paris auction was exclusively for Hergé's Tintin artwork - an edition of Explores to the Moon, signed by Hergé and astronauts Neil Armstrong, Buzz Aldrin and Michael Collins, was also auctioned for €34,000 (£27,000/$42,000).
The Tintin books were adapted into The Adventures of Tintin last year, by an all-star creative team including Steven Spielberg, Peter Jackson, Stephen Moffat and Edgar Wright, with plans already in place for a sequel.
Watch the Adventures of Tintin trailer below: