Warner Bros's latest clip from the animation sees the Caped Crusader use a hallucinogenic toxin similar to the Scarecrow's fear gas to foil two criminals.
The Dark Knight Returns is based on Frank Miller's classic four-part DC Comics miniseries, which sees an older Bruce Wayne returning from retirement.
Footage from the first instalment of the two-part adaptation debuted last month, following its debut trailer in July.
Peter Weller (RoboCop) stars as Batman and Ariel Winter (Modern Family) as Robin alongside a cast including Robert Paulsen and Maurice LaMarche.
Batman: The Dark Knight Returns - Part One will debut on Blu-ray and DVD on October 23 in the US. A UK release date is yet to be confirmed.