Marvel Comics has revealed that the milestone issue will be the last for ongoing series in its December solicitations.
Spider-Man has been expected to conclude for a while as part of the creative shake-up of the Marvel NOW! initiative.
The issue will be written by Dan Slott and illustrated by Humberto Ramos, with extra contributions by JM DeMatteis, Giuseppe Camuncoli, Jen Van Meter and Stephanie Buscema.
Amazing Spider-Man #699 and #699.1 will also ship in December.
Marvel NOW! will also see the end of the long-running Uncanny X-Men title, which was relaunched last year with a new #1.
A new Spider-Man title for the initiative is yet to be announced.