TV Guide confirms that the animated series' second season will debut with an hour-long special on January 21.
The series follows a young Peter Parker as he undergoes superhero training from S.H.I.E.L.D. head Nick Fury. Heroes from Marvel's Avengers Assemble will guest star in the second season.
Ultimate Spider-Man will see the title character team up with Nova, Iron Fist, Power Man and White Tiger to take on Lizard and Electro, as well as other members of the Sinister Six.
The show will air as part of Disney XD's Marvel Universe programming block, which will also introduce Marvel Comics Close-Up interstitials, hosted by Marvel chief creative officer Joe Quesada and Dylan Riley Snyder.
Ultimate Spider-Man will return to its regular Sunday morning time slot from January 27.