Doctor Octopus will be the hero of the upcoming Superior Spider-Man title.
Amazing Spider-Man #700 ended the long run with the death of Peter Parker, who was trapped in Otto Octavius's dying body.
Parker's former body is now inhabited by his long-time enemy, who also possesses all of Spider-Man's memories.
"This is the single most seismic shift in Spider-Man's history - and it's not something that will disappear anytime soon," said Marvel editor-in-chief Axel Alonso.
"When Spider-Man editor Steve Wacker and writer Dan Slott came to us with this story, it was clear that this was a long term plan crafted with the utmost love and respect for the character. This is the kind of story that will have fans up in arms - but also coming back to the comic stores to check out each new exciting issue."
The storyline caused an internet backlash - including death threats to writer Dan Slott - when it was leaked earlier in December.
Superior Spider-Man #1 will arrive on January 9.