The series finale shifted a quarter of a million copies during its initial run prompting Marvel Comics to sanction a second printing, which has added a further 50,000 to its total.
The publisher has now made around $2.5 million (£1.5m) from physical copies of the title alone, Bleeding Cool reports.
Amazing Spider-Man #700, written by Dan Slott and drawn by Humberto Ramos, brought the long-running series to an end to pave the way for Marvel NOW! title Superior Spider-Man.
Slott ended the saga with a game-changing twist that has seen him receive "death threats" on social networks.
Superior Spider-Man #1 was released this week.