Troy Baker, who has portrayed the expert marksman in Avengers Assemble, will provide the character's voice once again.
"Spider-Man is in a lot of trouble and S.H.I.E.L.D. brings in Hawkeye to sort of act as his bodyguard and then it just goes awry," Marvel Entertainment's head of television Jeph Loeb told TV Guide.
"We looked at those '80s movies with two guys who should never be partners, like Lethal Weapon, and that's what you get with Spider-Man and Hawkeye as they go up against a major super villain [The Beetle]. They may kill each other before they stop the guy."
Ultimate Spider-Man's second season debuted with an hour-long special on January 21 and has since reverted to its regular Sunday morning time slot on Disney XD.
The Hawkeye episode will air on February 10.