During filming of a recent episode of the CBS sitcom, the studio audience and showrunners Chuck Lorre, Bill Prady and Steve Molaro were surprised with a sudden live dance routine to Carly Rae Jepsen's 'Call Me Maybe'.
In between takes, the main cast members were sitting on the show's famous couch before the summer pop hit began playing in the background.
Seemingly confused at first, the cast including Kaley Cuoco (Penny), Kunal Nayyar (Raj), Simon Helberg (Howard), Mayim Bialik (Amy) and Melissa Rauch (Bernadette) join with the crew members in the dance.
Johnny Galecki (Leonard) also eventually joins in, while Jim Parsons (Sheldon) remains stood up in the background.
Following the dance, Parsons says in classic Sheldon mode: "Bazinga."
Cuoco posted the video on the show's official Facebook page, having come up with the idea. It was also choreographed by her sister Bri.
She wrote: "This literally was the best night of my entire life. A special thanks to the cast and badass crew, for going all in, and making it a night to remember!"
The video has almost attracted 15 million views on YouTube since it was uploaded on November 15.