BioShock 2 will be set ten years after the first game as players take on the role of the prototype version of the Big Daddy.
One of the major aspects from the original title was the moral quandary forced on players about whether to save the Little Sister characters or harvest them to gain the genetic manipulating ADAM.
Speaking to DS, executive producer Alyssa Finley said that these choices will be expanded for the second game, with players also being able to adopt the Little Sisters before having to protect them as they gather the ADAM.
She points out that this will bring a more "more nuanced relationship" with the characters, resulting in "critical decisions" for gamers.
"Do you save her by sending her to someone who will take care of her, or do you harvest her and take all the ADAM you just helped her gather?" asked Finley.
"And remember, you are not a stranger to her. She trusts you implicitly, what you do with that trust is one of the big choices in the game."
BioShock 2 will be released for Xbox 360, PC and PS3 on October 30.
Read our full interview with Finley this Sunday, May 24.