Poker Night At The Inventory will star Sam from Sam & Max, Tycho from Penny Arcade, The Heavy from Team Fortress 2 and Strong Bad from Homestar Runner, according to Kotaku.
"We've had the idea for some time of exploring the idea of what video characters do when they're not ‘on the clock' in the games we play," said Telltale CEO Dan Connors.
"We pitched the idea to friends in the industry and it really resonated. We all had a great time imagining what these guys would banter about while slinging cards - from there Poker Night at The Inventory was born."
Priced at $4.99 (£3), it will be available on PC and Mac via Steam later this year.
Telltale Games is also developing games for the Back To The Future and Jurassic Park licences.