Entitled The Da Vinci Disappearance, the pack will be released on PSN and Xbox Live at the beginning of March.
The DLC will cost 800 Microsoft Points (the PSN price is yet to be confirmed) and will contain new single-player and multiplayer content, Achievements and new characters Dama Rossa, the Knight, the Marquis and the Pariah.
The single-player campaign sees players attempt to rescue a kidnapped Leonardo Da Vinci from an underground cult called the Hermeticists.
According to CVG: "Ezio will embark on a relentless quest to recover stolen paintings in order to find Leo before it is too late, discovering two new locations and two new gameplay features along the way."
The new multiplayer map Alhambra is set in Spain's Albaicin of Granada and features multileveled indoor and outdoor areas.
The first of the new multiplayer modes is a team-based effort called Escort, which sees teams either protect or target a VIP, while the second mode is a ten-minute deathmatch called Assassinate.