Nintendo has announced that a variety of specialist chains, entertainment stores and supermarkets will take part, including GAME, HMV, Argos and Tesco.
The firm will also host VIP events in 13 stores across the country, which will provide discounted or free games to attendees on the night.
"We've been blown away by both retail and consumer reaction to Nintendo 3DS," said Nintendo UK general manager David Yarnton.
"With over 1,000 stores opening across the UK at midnight to launch the console, this has the potential to be one of our biggest launches ever in the UK."
It has previously been revealed that HMV will be the official launch partner for the system, with events in London and Liverpool. GAME will also release a list of stores opening at midnight.
The Nintendo 3DS will be available with 15 launch titles from March 25 in Europe.
The VIP stores in full:
Asda, Derby, Martin Luckwood, Asda Derby, Derby Road, Spondon, DE21 7LW
Tesco, Barr Hill, 15-18 Viking Way. Barr Hill, Cambridge, CB23 8EL
HMV, Liverpool 1, 5 South John Street, Liverpool, L1 8BU
Argos, Leeds Headrow, Unit 2, The Gantry, 22-26 The Headrow, Leeds, LS1 8EQ
Gamestation, Birmingham New Street, 138 New St, Birmingham, B2 4NS
Smyths Toys, Dublin, Jervis Street, Dublin 1
Xtra-Vision, Letterkenny, Unit 6-8. Letterkenny Shopping Centre Co. Donegal.
Gamestop, Dublin, Henry Street, Dublin
GAME, Glasgow Sauchiehall Street, 146 Sauchiehall Street, Glasgow, G2 2DH
GAME, Cardiff, 5 Grand Arcade, St Davids 2 Centre, Cardiff, CF10 2ER
GAME, Manchester, 124 Peel Centre, Trafford Centre, Manchester, M17 8BL
GAME, London, 100 Oxford Street W1
Gamestation, GST Hull, 59-60 Prospect Centre, Hull, HU2 8PW