The Outbreak pack will introduce prominent areas of the campaign into online multiplayer, according to Eurogamer.
Academy will be set in the play school section and sees players attempt to activate a decontamination system. Meanwhile, Concourse features a distress signal to locate somewhere inside a mall.
The Outbreak pack will be available on May 31 for Xbox 360 and June 3 on PlayStation 3.
Released earlier this year, Dead Space 2 outsold the original by two to one. The first DLC pack, Severed, was released in February.
> Read our PlayStation 3 review of Dead Space 2