The service, which is launching in Europe tomorrow, will also be joined by a separate Netflix application.
"Nintendo's short-form video service that delivers special video content for Nintendo 3DS systems will launch in North America later this summer," read a statement to Joystiq.
"In addition, Netflix members who have an unlimited plan starting at $7.99 (£5) a month and access to a broadband wireless Internet connection will soon be able to download a free Netflix application that allows them to watch movies and TV shows on their Nintendo 3DS systems at no extra charge."
The Nintendo Video application will be a free download to all users and will provide programming specifically for each region.
Content partnerships with Sky 3D and Eurosport will be available in the UK later this year.
Nintendo president Satoru Iwata declared in April that a video service would arrive on the 3DS shortly after the eShop.
The Nintendo Video app has already been released in Japan and is updated with new video content daily.