Legend of Zelda: Skyward Sword, Mario Kart 7, Super Mario 3DS Land, Metal Gear Solid: Snake Eater 3D and Kid Icarus: Uprising will all be playable at the expo, alongside an exclusive Professor Layton 4 trailer.
Furthermore, Disney has announced it will bring local multiplayer adventure Disney Universe to the event.
"From worlds inspired by Disney films to helping Nintendo's world-famous hero Link in Skyward Sword, we know gaming fans young and old will be in for a treat at this year's GAMEfest," said UK marketing director Anna-Marie Mason.
"Thanks to the involvement of the industry's top publishers, GAMEfest has something for the whole family to enjoy this September."
Modern Warfare 3 will see its first public showing at the Birmingham expo, it was announced last week.
Other attendees at the event include Sega, THQ, Sony Computer Entertainment, Electronic Arts and Microsoft.
GAMEfest will run from September 16 to 18 at Birmingham's NEC arena, with tickets now on sale for GAME reward card holders.
> Mario Kart 7: Preview
> Super Mario 3D Land: Preview
> Disney Universe: Preview
Watch trailers for Super Mario 3D Land and Mario Kart 7 below: