Assuming EA's upcoming role-playing game sells upwards of one million copies at £39.99, coupled with GAME's 20% market share, the retailer stands to lose £8m in total revenue, reports The Guardian.
"With new software margins of around 24% this could result in £1.5m-£2m of lost gross profit in the current year," explained analyst Mark Photiades.
The profit margin is further affected by the retailer's decision to offer £5 in reward points as compensation to customers who pre-ordered the title.
The withdrawal of Electronic Arts titles caused GAME's shares to plummet by 15%. US retailer GameStop has since expressed an interest in a takeover of the business as it aims to expand its European operations.
It is the latest in a series of problems for GAME. Earlier this year, it announced 35 store closures and that it is going to shut sister website gameplay.co.uk.
GAME recently received lender support to alleviate financial troubles following apparent concern by publisher EA.
The high street retailer's shares plummeted by 90% in 2011, despite £1 billion in annual turnover.
The retailer aims to turn its fortunes around by teaming with Gaikai to stream game demos.
> Everything must go: Is this the death of games retail?
Watch a trailer for Mass Effect 3 below: