Cross-platform play between PC and consoles was one of Valve's goals when starting development on the game.
The feature is getting cut to make frequent post-release updates possible on PC, the developer said.
"The beta has proved we want to update not just the beta, but the game itself post-launch frequently on the PC," Valve's Chet Faliszek told Joystiq.
"To do that we need to separate the platforms so one doesn't hamstring the other. So for that, we have removed the idea of cross-platform play - essentially make all platforms stronger by not mixing them."
Valve enabled cross-platform play in its last game, Portal 2, which allowed both PC and PlayStation 3 users to play together through Steam integration.
Counter Strike: Global Offensive will feature seven classic maps, five for the Bomb/Defuse mode and the other two for Hostage Rescue, with each map receiving graphical upgrades while leaving level layouts intact.
The game's beta launched last month on PC for users who completed a survey through Steam.
Counter-Strike: Global Offensive will release on PC, PS3 and Xbox 360 following the successful completion of its beta.
> Counter-Strike: Global Offensive interview - Valve on its latest game
> Counter-Strike: Global Offensive - Preview
Watch the Counter-Strike: Global Offensive trailer below: