American player Kathleen Henkel and Laura Rich from Cardiff played Solitaire Blitz on Facebook for 30 consecutive hours, totalling more than 1,500 hands.
Copyright: PopCapThe event, which took place simultaneously in London and New York, raised almost $93,000 (£59,000) for charity: water, which will use the money to provide 18 drinking wells to developing nations.
"Laura has showed resilience and an unshakeable passion for gaming over the last 30 hours, and she has certainly earned her place in the Guinness World Records family," said Gaz Deaves, Gaming Editor for Guinness World Records.
"Congratulations to Laura and her support team for an incredible record-breaking accomplishment."
31-year-old record breaker Laura Rich, meanwhile, said that it was an honour to use her love of Solitaire to raise so much money for charity.
"While achieving a world record today is an amazing feat, the thrill of helping earn nearly $100,000 for an organisation as great as charity: water is arguably just as exciting," she commented.
"To know that my love for a video game helped bring clean drinking water to thousands of people is an honour I will never forget."
PopCap Games also revealed plans to release Solitaire Blitz on iOS devices later this year.
Announced earlier this year, the aquatically-themed social card game launched on Facebook in March.
PopCap Games described Solitaire Blitz as an "underwater experience unlike any other", saying that it offers a new take on solitaire in a "fast-paced, social setting".
The game includes speed-based challenges, bonuses and gifts, all of which are topped off with a colourful aquatic art style.
> Feature: The Greatest Gaming Guinness World Records
Watch a trailer for Solitaire Blitz below: