The upcoming ice hockey game will feature former Team USA star Angela Ruggiero and Team Canada's Hayley Wickenheiser.
The athletes will be playable as legends, alongside male Hall of Fame stars Doug Gilmour and Jari Kurri.
It was also revealed that Philadelphia Flyers forward Claude Giroux has been selected as this year's cover athlete.
Giroux beat Nashville Predators goalie Pekka Rinne in the final of EA's Cover Vote campaign by less than a 1% margin.
NHL 13 will feature more realistic animation and improved online play.
It is backed by 'True Performance Skating', which allows the developers to introduce superior physics at faster speeds.
Through GM Connected support, up to 750 players can play together online in their own 30-team dynasty.
NHL 13 will be available for PS3 and Xbox 360 on September 11 in North America and September 14 in Europe. A demo is available now.
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Watch a trailer for NHL 13 below: