Titled 'Combat, Weapons and Tactics', the second part of the Inside Assassin's Creed series looks at the brutal realities of warfare during the American Revolution.
The video begins by looking at the game's weapons, before demonstrating the revamped fighting system and the motion capture process.
The trailer ends by focusing on the guerilla tactics used by Connor in the game and by the American soldiers of the era.
Officially unveiled in March, Assassin's Creed 3 will take place over a 30-year period in colonial America beginning in 1777.
It features a brand new lead named Connor, the offspring of an English father and Native American mother.
The game also introduces naval warfare elements, allowing players to participate in explosive battles out at sea.
Copyright: UbisoftPlayStation Vita spinoff Assassin's Creed 3: Liberation was announced at this year's E3 Gaming Expo.
It will support cross-play with the PlayStation 3 edition of Assassin's Creed 3, allowing players to unlock bonuses in both games by trading goods.
Assassin's Creed 3 and Liberation will be released for Xbox 360, PlayStation 3 and PlayStation Vita on October 31 worldwide.
A Wii U version is also in development and will be available during the system's launch window this holiday season.
The title is on course to break Ubisoft's record for the most pre-ordered game of all time.
> 'Assassin's Creed 3' E3 2012 preview: Eight anticipated features
> Digital Spy's 'Assassin's Creed 3' interview with Ubisoft
> Assassin's Creed 3 Liberation preview: Vita edition is dressed to kill
Watch the first part of the Inside Assassin's Creed 3 series below: