Narrated by Sir Hammerlock, the latest video serves as a beginner's guide to Borderlands, offering an introduction to the game's environment, characters and weapons.
The near seven-minute trailer offers tips on how to best use weapons and eliminate enemies, and also details the game's Relic system.
Relics are used to modify attributes, improving weapons and vehicles and increasing health. Class mods, meanwhile, improve specific skills, resulting in huge damage bonuses.
Borderlands 2 boasts a 58-hour campaign that will take players to previously unseen regions of the planet Pandora.
A number of special editions will be available, including the Borderlands 2 Deluxe Vault Hunter's Collector's Edition and the Ultimate Loot Chest Limited Edition.
The game will feature at least four pieces of post-launch DLC, which can be purchased individually or with the discounted Season Pass.
Borderlands 2 will be available for Xbox 360, PlayStation 3 and PC on September 18 in North America and on September 21 across Europe.
Gearbox recently created a retro mock-up of Borderlands 2 in the style of Smash TV.
Play the 16-bit Borderlands 2 promo game in your browser here.
> Read our latest 'Borderlands 2' interview with Gearbox Software
> Digital Spy goes hands-on with 'Borderlands 2'
Watch a trailer for Borderlands 2 below: