The competition, which challenged 3DS users with creating a 60-second film with a British summer theme, was won by 20-year-old student Nicholas Pratt.
Pratt's prize includes the chance to shoot a new 3D short film that will be shown before Nintendo's Gala screening of The Sapphires at the BFI London Film Festival on October 15.
For the re-shoot, Pratt will have access to professional equipment and be mentored by AV artist Stuart Warren-Hill and Senna director Asif Kapadia.
"I'm really excited to have this opportunity to get involved in the film industry!" Pratt remarked.
"It will be incredible to watch the film on the big screen with an audience and walk the red carpet - I'm pinching myself here!"
Three runners-up, meanwhile, will receive a pair of tickets to the BFI London Film Festival, as well as a membership to the organisation for one year.
Earlier this year, Nintendo launched a 3D photography competition, involving the likes of The Saturdays and Bill Oddie.
Nintendo had previously supplied the Louvre museum in Paris with 5,000 3DS gaming systems.
Gallery - view images of the 3DS photography competition below: