The pop youngster will join the Celebrity All-Stars team in the game, having apparently grown several feet taller for his virtual athletic debut.
An excited Bieber took to Twitter to share his in-game appearance, saying, "shoutout to @Ronnie2K for putting #TeamBieber in #NBA2K13 . I'm running the pt. #SWAGGY"
Bieber will play ball alongside the likes of JB Smoove, Wale, Bow Wow, Chamillionaire, Sean Kingston, Meek Mill, Vinny Guadagnino and Pauly D from Jersey Shore, Mac Miller and Brian Baumgartner on the Celebrity All-Stars team.
Celebrity All-Stars will be just one of many special teams in the game, including the 1992 US Dream Team.
2K Sports also confirmed Kinect support, enabling users to call certain plays, make substitutions and complain to the referee, a move that could lead to punishment.
It was recently revealed that Jay-Z will contribute to the "look and feel" of the title, hand-picking its soundtrack, designing the interactive menus and helping with the presentation.
The game releases for the Xbox 360, PS3, PlayStation Vita, Wii and PC on October 2 in North America. A European launch date is yet to be finalised.
NBA 2K13 also release for the Wii U. Listed for a "holiday" launch, it will likely be available within the first few months of the console's release.
Watch a trailer for NBA 2K13 below: