The latest developer diary video, titled Liberty Chronicles, offers a behind-the-scenes look at how Ubisoft recreated 18th century Louisiana for the game.
Announced at E3 2012, Assassin's Creed 3: Liberation stars Aveline, the series' first female protagonist, as she fights against the Spanish Empire's control over New Orleans.
It will support cross-play with the PlayStation 3 edition of Assassin's Creed 3, allowing players to unlock bonuses in both games by trading goods.
The video also reveals that during the course of the Vita spin-off's story Aveline will meet up and interact with Assassin's Creed 3 protagonist Connor.
At E3, Sony also revealed a new Vita bundle to launch with the game. It will include a new crystal white model of the handheld, a 4GB memory card and a copy of Assassin's Creed 3: Liberation.
Assassin's Creed 3: Liberation will release on October 31 worldwide, the same day Assassin's Creed 3 releases for Xbox 360 and PlayStation 3.
A Wii U version of Assassin's Creed 3 is also in development and will be available during the system's launch window this holiday season.
Watch the gameplay trailer for Assassin's Creed 3: Liberation below: