The box art reveals previously unconfirmed characters Green Lantern and The Joker as playable, the latter of which is prominently featured in the centre of the cover with his signature grin.
Yesterday NetherRealm Studios announced that Green Arrow will also join the roster, now bringing the total number of fighters up to 12 including Batman, Harley Quinn, Superman, The Flash, Wonder Woman, Catwoman, Solomon Grundy, Nightwing and Cyborg.
Announced in May, Injustice: Gods Among Us is said to have a deep original story, taking place in "a world where the lines between good and evil are blurred".
NetherRealm is developing the title following last year's success with Mortal Kombat. In 2008, the team also created Mortal Kombat vs DC Universe as Midway Games.
The developers have dropped the traditional three-round fighting system, opting instead for a "more cinematic" single-round system.
A Collector's Edition will be available, featuring steelbook casing, a character statue, two issues of a brand new DC comic book series and three character skins.
Injustice: Gods Among Us will be available on Xbox 360, PlayStation 3 and Wii U next April.
Watch the Injustice: Gods Among Us Green Arrow trailer below: