The video showcases the rich and breathing world of the stealth title, hinting at the sorts of characters that will populate the levels.
IO Interactive previously told Digital Spy that Hitman: Absolution will have a "more personal story" than previous games.
Lead producer Hakan Abrak added: "The other big difference is the technology. We've added new features and really upped the AI - because that's a core part of Hitman - to give those different, funny and weird stories within the story."
Hitman: Absolution will launch on Xbox 360, PlayStation 3 and PC from November 20 worldwide.
> Hitman: Absolution E3 preview: Agent 47 takes on the King of Chinatown
Watch a cinematic trailer for Hitman: Absolution below: