The latest Hitman trailer introduces Agent 47 as the "ultimate assassin".
The video showcases Agent 47's skills and looks at some of the new gameplay features, weapons and environments.
It is released alongside another trailer exploring the recently announced Hitman clothing line.
The Hitman: Absolution range of clothing, which includes hoodies and T-shirts, has been created by Trapstar and will be available in its flagship London store from November 20.
The first new Hitman game in six years, Hitman: Absolution will include new gameplay features, improved enemy intelligence and a new 'Instinct' mode that will help players blend into certain roles.
Described as the most ambitious game in the series yet, the stealth title features a "living and breathing world, where every moment can become a story".
IO Interactive told Digital Spy that Hitman: Absolution will have a "more personal story" than previous games.
Hitman: Absolution will be available on Xbox 360, PS3 and PC from November 20 worldwide.
Pre-order customers can also bag the Hitman Sniper Challenge mini-game, as well as the Hitman Absolution Deluxe Professional Edition.
> Hitman: Absolution E3 preview: Agent 47 takes on the King of Chinatown
Screenshot gallery - view Hitman Absolution images below: