The NASDAQ 100, which is updated every December, is a list of the top 100 non-financial stocks on the NASDAQ stock exchange.
Electronic Arts will be removed alongside eight other companies including Netflix and Research in Motion. Replacements include Google, Apple and Facebook.
Activision Blizzard is the only dedicated video game company remaining on the list.
"The securities being added to the NASDAQ 100 Index will join Facebook, Costco, Apple, Google and other household names that are leading the new economy forward," revealed NASDAQ executive VP John L Jacobs.
"Our objective re-ranking process ensures the NASDAQ 100 remains a relevant investable index that is the underlying benchmark for about 7,100 products in 22 countries with a notional value of about $1 trillion."
Electronic Arts's stock has been on a decline this year, reaching $15.42 (£9.76) per share yesterday, compared to nearly $25 (£15.89) per share last November.
Back in April of this year, rumours circulated that South Korean publisher Nexon was in talks to purchase EA, causing the American publisher's stock prices to see a temporary rise. The talks turned out to instead be a deal for Nexon to publish Fifa Online 3 in Korea.
Recent releases such as FIFA 13 have proven successful for Electronic Arts, with the football game selling 7.4 million copies in four weeks.
> Digital Spy's 'FIFA 13' review: 'Another trophy-winning instalment from EA'
Watch a trailer for FIFA 13 below: