The video highlights Hunter Mode, a co-operative multiplayer mode for up to four players both locally and online.
In Hunter Mode each player will control a different coloured Luigi as they capture ghosts and make their way through the Scarescraper tower.
Players will be able to set the difficulty and number of floors, choosing between 5, 10 or 25 floors.
Bonuses and upgrades can be earned by working together as a team, and players will even be able to revive fallen teammates to keep their team intact.
Earlier this week multiplayer was spotted as a feature in the game's official description, which was originally thought to be local only. With today's announcement, Nintendo has confirmed that the co-operative mode will be playable online as well.
Gallery - images of Luigi's Mansion: Dark Moon below:
Copyright: NintendoLuigi's Mansion: Dark Moon is the Nintendo 3DS follow-up to the GameCube launch title. The game will feature multiple mansions for Luigi to explore, filled with ghosts to capture and puzzles that will unlock new rooms.
Luigi will use a vacuum called the Poltergust 3000 to capture ghosts, and can also use wind to interact with the environment for puzzle solving.
The game will also take advantage of the 3DS's gyroscope, which can be used to aim Luigi's flashlight and vacuum.
Nintendo recently confirmed that the game will release on March 24 in North America and an unspecified date in March for Europe.