The 'From Ashes' trailer, which was teased earlier this week, deals with the moment Kratos loses his family.
Described by Sony Santa Monica as the "biggest production we've ever undertaken for a God of War commercial", the advert features a special arrangement of Ellie Goulding's 'Hanging On'.
The trailer releases alongside new God of War screens and 30 minutes of gameplay footage taken from the opening chapter of the game.
God of War: Ascension will take place before the events of the original trilogy, presenting a more human side of protagonist Kratos, set in a time before his desire for vengeance consumed him.
It will be the first entry in the series to include multiplayer support, enabling up to eight players to compete in a variety of game modes and customise their characters.
Sony recently confirmed that a demo for The Last of Us would be available to customers who purchase God of War: Ascension.
However, the sampler will not be available at launch, but will be released at a later date.
God of War: Ascension will be released for PlayStation 3 on March 12 in North America and on March 13 in Europe.
Screenshot gallery - God of War: Ascension images: