The first episode of the second series, 'The Gunpowder Plot', will be available to download free from the Doctor Who website from October 31.
The Royle Family's Ralf Little will voice the role of Guy Fawkes, alongside Phil Daniels, Emilia Fox and Chris Johnson.
Little said: "It is a way of bringing the story alive to, probably, millions of kids who are aware of the name Guy Fawkes because of November the 5th, but not actually really understand what the historical significance is behind it."
Written by Phil Ford, 'The Gunpowder Plot' sees The Doctor, Amy and Rory battle two old Doctor Who enemies in London in 1605.
The new series will see several gameplay enhancements, including non-linear routes, more sophisticated puzzles and a more complex lighting system.
The BBC recently teased that the next edition of Doctor Who: The Adventure Games would be the best yet.
> Read our preview of Doctor Who: The Adventure Games' Christmas episode 'Shadows Of The Vashta Nerada'