The sports broadcaster will air at least two games per week from Italy's top division, as well as a selection of Coppa Italia games.
It will also broadcast at least two matches each week from the MLS regular season and the playoff games.
After a successful debut season last year, ESPN will continue to broadcast ESPN Kicks - Serie A recapping all of the weekend's goals, as well as weekly preview show, Total Italian Football.
ESPN EMEA vice president of TV channels Jeroen Oerlemans said: "The renewal of these agreements is an excellent result for all football fans in the UK, giving them access to live and exclusive coverage of two of the world's most talked-about leagues. We are building an excellent portfolio of live football and sport on ESPN."
For the 2010/11 season, ESPN will also air 23 English Premier League games and 30 Scottish Premier League ties, along with FA Cup matches and action from the Bundesliga, Dutch Eredivisie, Russian Premier League and the UEFA Europa League.
Yesterday, ESPN secured a deal to broadcast 100 archive Premier League matches every season on the ESPN Classic channel and associated video on-demand platforms.