Owners of The Daily Telegraph, the Daily Mail, The Guardian and The Mirror have signed a petition submitted to the business secretary Vince Cable urging him to consider blocking Murdoch's £8 billion bid to take full control of pay-TV operator Sky.
The media groups are concerned that the integration of Sky's television platform with the News International titles, including The Times and The Sun, could have "serious and far-reaching consequences for media plurality".
The signatories argue that if Murdoch is allowed to complete the takeover, his media empire would have a combined turnover of £7.5bn compared with the BBC's £4.8bn.
Joining them in signing the petition is Channel 4 chief executive David Abraham, BT chief executive Ian Livingstone and BBC director general Mark Thompson, who recently expressed his grave concern about the proposed deal.
The letter also includes a brief from law firm Slaughter & May outlining the legal circumstances for Cable to intervene in the takeover.
In June, News Corp failed in a 700p per share bid to buy the 61% of Sky that it does not already own. However, the two parties agreed to keep the lines of negotiation open.
News Corp is soon expected to formally notify the European Commission about its intention to mount a takeover of the satellite broadcaster.
The move will kick off a regulatory process that could result in Cable referring the deal to media regulator Ofcom for further investigation on grounds of public interest.
Yesterday, Lord Fowler - the former chairman of the House of Lords select communications committee - called on Cable to intervene in the takeover as it is a "matter of legitimate public concern".