According to The Guardian, the document contains questions based on objections raised by rival media firms during Ofcom's investigation into the impact of the takeover on media plurality in the UK.
News Corp will have to respond to the questions before Christmas, as Ofcom must submit a final report to business secretary Vince Cable by December 31.
Ofcom chief executive Ed Richards is personally overseeing the inquiry due to the highly sensitive nature of News Corp's bid to acquire the 61% of Sky that it does not already own.
Rupert Murdoch's News Corp argues that the deal is motivated purely by financial considerations, but opponents claim that it will create an overly dominant media player in Europe.
After Ofcom submits its final report to Cable, the business secretary will have to decide what action to take, including a possible further review at the Competition Commission. However, senior sources within News Corp remain confident that the takeover will ultimately be given the go-ahead.
News Corp also recently submitted a potential remedy solution to the European Commission in an attempt to smooth through the organisation's own review of the Sky bid.