The new reality TV series follow the lives and loves of a group of young lesbians, including former Big Brother housemate Shabby Katchadourian, working at London's Candy Bar.
A series of on-air promos for the show featured close-up images suggesting sexual activity, such as a woman kneeling down in the lap of another female, as the voiceover said: "Pussy... loving... ladies."
However, the wider shot then revealed that the woman was actually stroking a cat in the other's lap, while the voice said: "Well, what were you expecting?", before a plug for Candy Bar Girls.
In its weekly Broadcast Report, Ofcom confirmed that it has launched an investigation into the promos after receiving 14 complaints from viewers about their transmission during the day.
The media regulator will investigate whether the films breached the Broadcasting Code in terms of their scheduling and potential to cause harm or offence.
The Advertising Standards Authority has also received a complaint about the broader Candy Bar Girls advertising campaign on Channel 5.
Elsewhere in Ofcom's Broadcast Report, the regulator confirmed that it has received 29 complaints about Sri Lanka's Killing Fields, aired on Channel 4 on June 14.
The documentary contained footage that Channel 4 admitted was "probably the most horrific the channel has ever shown", including harrowing scenes of atrocities committed in Sri Lanka's 26-year civil war.
Ofcom will now launch an investigation into the complaints, but the programme's 11pm scheduling and clear warnings given at its start and at the end of every advertising break suggest that the regulator will not take any action.