The message was recorded by the Queen - only the second monarch to celebrate 60 years on the throne - ahead of the star-studded concert at Buckingham Palace on Monday (June 4) night.
It will be shown at 6pm on radio and television in the UK and across the Commonwealth, after the final celebrations of the historic Jubilee.
But the day is tinged with sadness for the Queen as Prince Philip remains in hospital with a bladder infection.
Around 2,000 guests are to gather at St Paul's to give thanks for the Queen's "loyal service and commitment".
Prime Minister David Cameron will lead a large representation from the government and the Archbishop of Canterbury, Dr Rowan Williams, will give the sermon.
The Queen will then attend a reception at Mansion House - the official residence of the Lord Mayor of London - followed by a carriage procession through Whitehall and, weather permitting, an RAF flypast.
BBC big screens will broadcast the service, carriage procession and appearance by the Queen on the palace balcony live in 22 locations around the UK.
Photo gallery - Diamond Jubilee concert at Buckingham Palace:
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