The 38-year-old DJ revealed live on-air during Wednesday's (July 11) edition of the programme that he will be leaving the show in September after eight years.
"I know some kids wanted to be a professional footballer or a fireman but not me, I'm a geek," he stated. "I wanted to be on the radio.
"I wanted the biggest radio show you can get and eventually, I got it. And for the last eight and a half years, I've had the best time of my life."
Moyles has since taken to Twitter and admitted to his 2.5 million followers that he felt "humbled" by the reaction he received.
"I would like to say Thank You for all the comments about me, the team and the show," he wrote.
"Really appreciate it and have been quite humbled. Thanks."
Nick Grimshaw has been confirmed as the new Radio 1 Breakfast Show host.
Recalling the moment station controller Ben Cooper revealed the news to him, Grimshaw said: "I thought I was in trouble. So I went in and I was complaining that I got up early.
"And he's like, 'Well, you're going to have to start getting up a little bit earlier'."
Moyles will go on to play King Herod in the upcoming arena tour of Jesus Christ Superstar, which also stars Mel C and Tim Minchin.
Photo gallery - Chris Moyles's career in pictures:
Copyright: Rex Features