Companies House filings have revealed that the media mogul also stepped down from the boards of Times Newspaper Holdings and News Corp Investments in the UK last week.
The latest development has fueled rumours that Murdoch is planning to sell the newspaper group, The Daily Telegraph reports.
News Corporation called the move "nothing more than a corporate housecleaning exercise prior to the company split".
It was announced back in June that News Corporation will split its business into two separate companies - one containing the lucrative entertainment assets and the other News Corp's troubled publishing operations.
"I am 100% committed to the future of both the publishing and media and entertainment businesses and, if the board ultimately approves a separation, I would serve as chairman of both companies," Murdoch said at the time of the announcement.
He has also quit a number of News Corp's US boards, details of which are yet to be disclosed by the US Securities and Exchange Commission.
James Murdoch resigned from various directorships at News International last November.
He later stepped down as executive chairman of BSkyB and executive chairman of News International newspapers in the UK.
News Corp sources claim that the Murdochs could sell their stake in the publishing division following the split, using the equity to fund a buyout of the film and entertainment division.