Circulation of the children's comic hit 2 million copies a week in its heyday, but slumped to below 7,500 last year as kids increasingly turned to video games and social networking for their entertainment.
Despite various efforts to rejuvenate it for the modern age, including special celebrity editions, The Dandy could not match the performance of its sister title The Beano, which sells around 38,000 copies per week.
DC Thomson, publisher of The Dandy, has now confirmed that the final print issue of the comic will come out on December 4, coinciding with its 75th anniversary.
The special edition of The Dandy will include a reprint of the very first Dandy comic from 1937, but it will also be the final print outing for the title.
Ellis Watson, the chief executive of Dundee-based DC Thomson, said: "On December 4, we'll publish our 75th-anniversary edition - and now that the cat's out of the bag - I can confirm that this will be our last print edition. It's what comes online then that will set the tone for the next 75 years."
The publisher was forced to deny earlier this week that The Dandy was to close, after reports of its forthcoming demise were leaked in the press.
Watson said that he was "absolutely livid" about an internal leak that had led to The Dandy's plans reaching the media early.
In a statement, Watson confirmed that security had been put into "lockdown" at the publisher following the leak.
"I'm absolutely livid that we've had an internal leak about what's in the pipeline for one of our most popular titles," Watson said.
"I've closed down loopholes to ensure much tighter internal security. We've heard about people trying to find out the plans by hacking into www.dandy.com so we've taken it all offline and moved into core project offices. We're in lockdown.
"Dan has certainly not eaten his last cow pie. All of The Dandy's characters are just 110 days away from a new lease of life."
The Dandy Annual will continue to be printed and the 2013 annual is already on the shelves.
The Cartoon Museum in London is currently preparing an exhibition marking the 75th anniversary of the first edition of The Dandy, which went on sale in 1937, priced at 2p with a free whistle.