Marking the "culmination of an extraordinary British summer", the classical music showpiece will be screened live in 3D for the first time at Odeon cinemas in the UK on September 8.
Eight specialist cameras will be used to capture the 3D footage, intended to give viewing audiences "the best seats in the house and provide an immersive experience".
The cameras include a key position in front of the conductor and a remote camera within the orchestra that rotates at 180 degrees (including pan and tilt) to make viewers feel like they are amongst the musicians.
Acting as the traditional finale of the BBC Proms, the Last Night caps two months of music-making at the Royal Albert Hall, aired live on BBC One, BBC Two, and BBC Radio 3.
Soloist Nicola Benedetti will perform Bruch's popular Violin Concerto No 1 in G minor on the night and Maltese tenor Joseph Calleja is to lead the traditional singalong including Jerusalem and You'll Never Walk Alone.
"Bringing the Last Night of the Proms to cinemas for the first time is a very exciting way of us reaching more people across the UK to celebrate the end of this extraordinary summer," said Roger Wright, the director of the BBC Proms.
"And delivering the Last Night of the Proms in 3D means audiences will be able to experience the event like never before."
Audiences will be able to watch the Last Night of the Proms in 3D at Odeon cinemas in:
Trafford Centre Manchester
Tickets to the screenings can be purchased at bbc.co.uk/proms.
The Last Night of the Proms in 3D will also be available to watch for anyone who has a 3D TV set and can receive the BBC HD Channel, regardless of which digital TV provider they use.
This forms part of the BBC's two-year trial of 3D production, which has recently seen live coverage of Wimbledon and the London 2012 Olympics.
Yesterday (August 19), the corporation aired Planet Dinosaur 3D: Ultimate Killers - a 3D programme adapted from the multi-award winning BBC One series.