The DJ was joined by his regular team members including 'Comedy' Dave Vitty, Aled Haydn-Jones and Dominic Byrne for a special show hosted at BBC Radio Theatre in Broadcasting House.
Presented in front of a live studio audience and broadcast live on the BBC Red Button, Moyles and the team were joined by a selection of celebrity guests and friends of the show.
Ant & Dec surprised the host by presenting him with the 'big red book' for a special edition of This Is Your Life.
The duo said: "In 2004, you had been a presenter on Radio 1 for seven years. But you made no bones about the fact that you didn't want to be there. The breakfast show is where you wanted to be."
Gary Barlow, Davina McCall, Fearne Cotton, Billie Piper and previous breakfast show host Sara Cox appeared as guests to wish Moyles well.
Cheryl Cole also provided a video message, saying: "I just wanted to say good luck with everything you do in the future. I'll never forget the memories we shared on the mountain [Kilimanjaro for Comic Relief]. Best wishes from me, and happy new chapter."
Show favourite and Carpark Catchphrase presenter Roy Walker appeared, saying: "It's been incredible, Chris is the most honest guy I've ever met. It was the best fun of my whole life, it was class."
James Corden also made a heartfelt speech, saying about the show: "You've got a group of friends every morning. It doesn't matter where you are, you've got these friends who never exclude you every morning.
"The more you listen, the more you become part of it, that's the reason we've stuck with it for so long. It's going to be like losing a group of mates in the morning that you happen to be in the car with."
He added: "It has to end at some point, we should just be happy with the fact that there might not be anybody else who will do the breakfast show this long again, it's a really special thing."
Pixie Lott later performed an orchestra reworking of her hit 'All About Tonight', titled 'All About Chris Moyles'.
Chris Moyles will present his last ever BBC Radio 1 breakfast show tomorrow morning (September 14) from 6.30am. Nick Grimshaw will take over from Monday, September 24.
Chris Moyles's career in pictures:
Copyright: Rex Features