The DJ has fronted the coveted slot on the music station for a record eight years and eight months, since launching it in January 2004.
He finished the show with a song and dance number that utilised his famous 'Cheesy Song' jingle.
After Moyles had appeared to have ended the show - which was being broadcast live on the BBC's Red Button - he turned round to the camera and began lip-synching to a farewell song.
The final shot saw Moyles saying: "Goodbye Great Britain", as he walked out of the BBC's, Broadcasting House in London.
> McFly sing 'Star Boy' goodbye to Chris Moyles - video
Each team member gave a heartfelt goodbye message to their loyal listeners, with newsreader Dominic Byrne saying: "I started this show when my boy Finn was 6 months old. Now he's 9, and we've had two other children since then. It's been a gigantic part of our lives as a family and for me personally.
"I feel very proud of what we've done and I don't think it will be done again. I feel very, very proud and thanks to everyone who's listened. I will miss it and genuinely from the bottom of my heart, thanks very much."
'Comedy' Dave Vitty said: "I just want to say thanks very much to everybody who's listened over the years. I've worked with great people, and have done some great things. Without you, we wouldn't be here."
Producer Aled Haydn-Jones added: "I was 6 when I played my first radio show to my gran, I was 15 on the day when I got my first radio show at hospital radio, and throughout the whole time, all I wanted to do was work on the breakfast show. I never thought I'd be doing it for nine years.
"I just want to say I love you guys and I love the audience and what an amazing place radio has been and Radio 1 still is."
Finally, Chris Moyles said his own goodbyes to the listeners, saying: "I've had best time ever, I've had a few lows, but mainly a gazillion highs doing this show. It has been my dream doing it and has been so much better than it could be.
"Nick [Grimshaw], have the best time. It's the best job in the world, you get a lot of grief with it, but it's worth going through that. Nick might have a rough time at the beginning but I hope they give him a try.
"It's been awesome, thanks to the team who've been great, and the listeners who are more than just people that listen to the show."
He added: "I'm genuinely so excited and happy to have got here without being marched out of the building. Thanks to everyone who's worked on the show and all the people who've supported show. We're a tough act to follow, it's great. So long and thanks for all the fish."
The final episode followed a special farewell show yesterday (September 13) that featured guests including Gary Barlow, Ant & Dec and James Corden.
Nick Grimshaw will take over the breakfast show on BBC Radio 1 from Monday, September 24 at 6.30am.
Chris Moyles's career in pictures:
Copyright: Rex Features